ABSTRACT
The levels of some important drug activating and detoxyfying enzymes were estimated in the livers of Swiss mice treated with a local brand of country liquor. Following liquor ingestion in male mice elevated levels of hepatic cytochrome P-450 were observed, while female mice did not show this. Cytochrome b5 levels remained unchanged. Similarly in male mice, increase in hepatic reduced glutathione levels were obtained while in female mice, decrease in this was observed. The activity of glutathione S-transferase was not changed. It is suggested that the increases in cytochrome P-450 and in hepatic reduced glutathione may be important determinants in carcinogenecity of the country liquors.
Subject(s)
Alcoholic Beverages/adverse effects , Animals , Cytochrome P-450 Enzyme System/drug effects , Cytochromes b5/drug effects , Female , Glutathione/metabolism , Glutathione Transferase/drug effects , India , Male , Mice , Microsomes, Liver/drug effects , Sex FactorsABSTRACT
Epidemiological studies reveal that alcohol consumption is a risk factor for the cancer of the mouth, larynx, esophagus and various other organs. Of the various alcoholic beverages consumed in India, country liquors are widely consumed and that too by the economically weak section of the society. The present paper describes the experiments designed to investigate the effect of one brand of country liquor from Maharashtra State, India (which was found to be more potent in our earlier mutagenicity studies) for its carcinogenicity in two strains of mice and Syrian golden hamsters. The experimental animals received 10% liquor in drinking water from 2 months of age for 16 months. One percent ethanol treated animals served as positive controls. Together with long term bioassays, the transplacental carcinogenic effect of country liquor in the offspring of treated mothers, as well as in the breeders themselves was also investigated. Pregnant mothers were fed 10% liquor through drinking water from 12th day of gestation till weaning of the progeny. Then offspring were allowed to live without further treatment and mothers continued to get liquor treatment. In long term bioassays, liquor caused 22% total tumor incidence in male BALB/c mice and 28% in male Swiss mice. In female Swiss mice and in hamsters, liquor did not show any pronounced effect on tumor incidence. Similar negative results were obtained in case of offspring of treated mothers, but the offspring of liquor treated mothers had higher mortality prior to weaning as compared to those of untreated mothers.